β-Nicotinamide adenine dinucleotide, reduced form, disodium salt (NADH-Na2, CAS 606-68-8) is the reduced coenzyme form of NAD and the principal carrier of reducing equivalents in enzymatic redox reactions. PolyBlueChem supplies NADH disodium salt as a high-purity powder, assay ≥98%, for in vitro diagnostic (IVD) reagent manufacturing, biocatalysis and life-science research, with per-batch COA and SDS.
Specifications
| Property | Specification |
|---|---|
| Product form | Powder (freeze-dried / spray-dried, non-sterile) |
| Assay (HPLC) | 98.0% min |
| Appearance | White to slightly yellow powder |
| Water content | Confirmed per batch on the COA (hygroscopic) |
| Heavy metals | Confirmed per batch on the COA |
| Solubility | Freely soluble in water; soluble in aqueous buffer at pH 7-8 |
| Storage | Sealed, -20 °C, protected from light and moisture |
Grade offered: biotechnology grade (non-sterile). Batch-specific values for water content, heavy metals and residual solvents are stated on the certificate of analysis supplied with each lot.
Salt form and grade selection
| Form | CAS | When it is the right choice |
|---|---|---|
| NADH disodium salt (this product) | 606-68-8 | Standard choice for enzyme-based assays and biocatalysis; highest water solubility, pH-neutral in solution |
| NADH free acid | – | Only where sodium must be excluded from the reaction system; lower solubility, slower dissolution |
| NAD (oxidised) | 53-84-9 | When the assay measures NADH formation and the oxidised form is the substrate |
| NADPH tetrasodium salt | 2646-71-1 | When the coupled enzyme is NADP(H)-specific (e.g. glutathione reductase, P450 systems) |
Basic Information
| CAS number | 606-68-8 |
| Chemical name | β-Nicotinamide adenine dinucleotide, reduced form, disodium salt (dihydronicotinamide-adenine dinucleotide, disodium salt) |
| Formula / MW | C21H27N7Na2O14P2 / 709.4 g/mol (anhydrous basis) |
| PubChem CID | 2724710 |
| Type | Pyridine nucleotide coenzyme, reduced form |
| Absorption maximum | 340 nm (molar absorptivity ≈ 6.22 × 103 M-1cm-1) |
Applications
- In vitro diagnostic reagents – the co-substrate in enzyme-coupled IFCC methods (ALT, AST, LDH, GLDH, γ-GT and similar), where the decrease in absorbance at 340 nm is proportional to analyte concentration.
- Biocatalysis – reducing equivalent for ketoreductases (KRED), alcohol dehydrogenases and ene-reductases; usually supplied with an NADH regeneration system (glucose/glucose dehydrogenase, formate/formate dehydrogenase or isopropanol/ADH).
- Research – NAD+ metabolism, mitochondrial function and redox biochemistry studies.
NADH is a biochemical reagent, not a finished drug or food ingredient. It is not offered for therapeutic or supplement claims.
Handling, storage and documentation
- Moisture and oxygen sensitive – weigh quickly, keep containers sealed, and prepare solutions freshly. Aqueous NADH oxidises to NAD+, faster at acidic pH and under light.
- Recommended solution practice: dissolve in phosphate or Tris buffer at pH 7.0-8.0 and keep on ice; do not autoclave.
- Per-batch COA and SDS supplied; an assay method statement and third-party testing are available on request.
- Packaging: small-pack foil/bottle for reagent use, bulk pack for manufacturing; quantities and packaging are agreed against the required grade.
Related products
- NAD (CAS 53-84-9) – 85% min solution, 98% min and 99% min powders
- NADPH free acid (CAS 53-57-6) – reduced form
- NADPH tetrasodium salt (CAS 2646-71-1) – assay 95% min
- NADP disodium salt (CAS 24292-60-2) – 75% min solution and 98% min powder
- NADP free acid (CAS 53-59-8) – assay 98% min
Further reading
- NADH (CAS 606-68-8): stability, diagnostic assays and biocatalytic use
- NADH vs NADPH: choosing a reducing coenzyme
- NAD, NADH, NADP and NADPH: coenzyme forms, salt types and bulk supply
PolyBlueChem supplies β-NADH disodium salt (CAS 606-68-8), assay 98.0% min, with per-batch COA and SDS and FOB/CIF delivery. Contact us with your quantity, assay requirement and destination.
