β-Nicotinamide adenine dinucleotide phosphate, reduced form, free acid (NADPH, CAS 53-57-6) is the reduced coenzyme of the phosphorylated pyridine nucleotide series, supplied as a sodium-free powder. PolyBlueChem supplies NADPH free acid, assay ≥98.0%, biotransformation grade, with per-batch COA and SDS.
Specifications
| Property | Specification |
|---|---|
| Product form | Powder (free acid, sodium-free) |
| Assay | 98.0% min |
| Grade | Biotransformation / research grade |
| Appearance | White to pale yellow powder |
| Sodium content | None added (free acid) – lowest sodium load of the NADPH forms |
| Water content | Confirmed per batch on the COA (hygroscopic) |
| Solubility | Soluble in water; slower to dissolve than the sodium salts – pre-dissolve and adjust pH before dosing |
| Storage | -20 °C or below, sealed, protected from light, oxygen and moisture |
Free acid or tetrasodium salt is a sodium-load decision, not a potency decision. Both carry the same coenzyme per mole: 745.4 g of free acid equals 833.3 g of tetrasodium salt. Convert your protocol in molar terms before switching forms.
NADPH free acid vs tetrasodium salt
| Criterion | Free acid (this product) | Tetrasodium salt (2646-71-1) |
|---|---|---|
| Formula / MW | C21H30N7O17P3 / 745.4 | C21H26N7Na4O17P3 / 833.3 |
| Sodium per mole | 0 | 4 |
| Dissolution | Slower; needs pH adjustment (the free acid solution is acidic) | Fast, near-neutral in solution |
| Ionic strength of the final mix | Lowest – useful in high-salt or conductivity-sensitive reactions | Adds four sodium per mole – check before use in a tight ionic-strength window |
| Typical use | Reference standards, sodium-sensitive enzyme systems, in-house buffer preparation | Direct dosing into biocatalytic reactions |
Basic Information
| CAS number | 53-57-6 |
| Chemical name | β-Nicotinamide adenine dinucleotide phosphate, reduced form (NADPH, dihydronicotinamide-adenine dinucleotide phosphate) |
| Formula / MW | C21H30N7O17P3 / 745.4 g/mol |
| PubChem CID | 5884 |
| Type | Pyridine nucleotide coenzyme, phosphorylated, reduced form |
| Absorption maximum | 340 nm (used for spectrophotometric quantification) |
Applications
- Biocatalysis – reducing agent for NADPH-dependent oxidoreductases: P450 monooxygenases, ketoreductases, glutathione reductase, dihydrofolate reductase; normally paired with a regeneration system rather than dosed stoichiometrically in bulk.
- Enzymatic analysis – preparing calibration standards where no added sodium is wanted.
- Research – oxidative stress, glutathione metabolism and redox signalling.
Supplied as a biochemical reagent; not offered for therapeutic or supplement claims.
Handling, storage and documentation
- NADPH oxidises in air and light, faster in acidic solution – dissolve fresh, keep on ice and use the same day.
- Aliquot the bulk pack; avoid repeated freeze-thaw cycles.
- Per-batch COA and SDS supplied; assay method statement and third-party testing available on request.
- Packaging from laboratory packs to bulk, agreed against your process.
Related products
- NADPH tetrasodium salt (CAS 2646-71-1) – assay 95% min, fast-dissolving
- NADP free acid (CAS 53-59-8) – the oxidised form
- NADP disodium salt (CAS 24292-60-2) – 75% min solution and 98% min powder
- NADH disodium salt (CAS 606-68-8) – the non-phosphorylated analogue
- NAD (CAS 53-84-9) – solution and powder grades
Further reading
- NADH vs NADPH: choosing a reducing coenzyme
- NADP free acid, monosodium or disodium: choosing the salt form
- NAD, NADH, NADP and NADPH: coenzyme forms, salt types and bulk supply
PolyBlueChem supplies β-NADPH free acid (CAS 53-57-6), assay 98.0% min, with per-batch COA and SDS and FOB/CIF delivery. Contact us with your quantity, assay requirement and destination.
